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close this bookHealth and Environment in Sustainable Development - Five Years after the Earth Summit (WHO, 1991, 128 p.)
close this folderPart II. Essential media and reagents for isolation and identification of clinical pathogens
close this folderPriority grading of pathogens, culture media, and diagnostic reagents
View the document(introduction...)
View the documentBlood culture
View the documentCerebrospinal fluid specimens
View the documentUrine
View the documentStool culture
View the documentLower respiratory tract
View the documentUpper respiratory tract
View the documentUrogenital specimens for agents of sexually transmitted diseases
View the documentPus and exudates
View the documentList of recommended culture media and diagnostic reagents for the intermediate microbiological laboratory

Urine

Common pathogens

Pathogens with high priority

Escherichia coli
other Gram-negative rods
enterococci
Staphylococcus saprophyticus

Pathogens with intermediate priority

Pseudomonas and other non-fermenters
other staphylococci

Pathogens with low priority

Candida albicans
Mycobacterium tuberculosis

Isolation media and diagnostic reagents

Isolation and quantitative media

Priority code

brolacin agar (can be replaced by purple lactose agar, MacConkey agar without crystal violet, or eosin methylene blue agar)

1

Identification media and reagents


bile-aesculin agar (for enterococci)

2

novobiocin (5-mg) disc for differentiating coagulase-negative staphylococci

3

b-glucuronidase tablet (PGUA) for identification of E. coli

2

for Gram-negative rods:

1


Kligler iron agar

1


motility-indole-urease medium (MIU)

1


Kovac’s reagent for indole

1


oxidase reagent

1


ONPG test

2


Simmons’ citrate agar

2


cystine decarboxylase broth (Mr)

2

for staphylococci and enterococci:



coagulase plasma

1


catalase test (H2O2)

1


oxidase reagent

1


hydrogen generator for anaerobic jar

2