 | Health and Environment in Sustainable Development - Five Years after the Earth Summit (WHO, 1991, 128 p.) |
 |  | Part II. Essential media and reagents for isolation and identification of clinical pathogens |
| | Priority grading of pathogens, culture media, and diagnostic reagents |
|
|
Urine
Common pathogens
Pathogens with high priority
Escherichia coli
other Gram-negative rods
enterococci
Staphylococcus saprophyticus
Pathogens with intermediate priority
Pseudomonas and other non-fermenters
other staphylococci
Pathogens with low priority
Candida albicans
Mycobacterium tuberculosis
Isolation media and diagnostic reagents
|
Isolation and quantitative media |
Priority code | |
|
brolacin agar (can be replaced by purple lactose agar, MacConkey agar without crystal violet, or eosin methylene blue agar) |
1 | |
|
Identification media and reagents | | |
|
bile-aesculin agar (for enterococci) |
2 | |
|
novobiocin (5-mg) disc for differentiating coagulase-negative staphylococci |
3 | |
|
b-glucuronidase tablet (PGUA) for identification of E. coli |
2 | |
|
for Gram-negative rods: |
1 | |
| |
Kligler iron agar |
1 |
| |
motility-indole-urease medium (MIU) |
1 |
| |
Kovac’s reagent for indole |
1 |
| |
oxidase reagent |
1 |
| |
ONPG test |
2 |
| |
Simmons’ citrate agar |
2 |
| |
cystine decarboxylase broth (Mr) |
2 |
|
for staphylococci and enterococci: | | |
| |
coagulase plasma |
1 |
| |
catalase test (H2O2) |
1 |
| |
oxidase reagent |
1 |
| |
hydrogen generator for anaerobic jar |
2 |
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