Transport media

Priority code

Cary-Blair medium (for all pathogens)

1

buffered glycerol saline (not for Vibrio and Campylobacter)

2

Enrichment media

selenite F broth

2

alkaline peptone water

2

Isolation media

MacConkey agar (with crystal violet)

1

deoxycholate citrate agar (can be replaced by Salmonella-Shigella agar)

1

TCBS agar (can be replaced by Monsur’s TTG agar)

1

Campylobacter medium: Columbia agar base or any blood agar base with lysed blood and antibiotic supplement

2

Clostridium difficile selective medium

3

Preliminary identification media and reagents

Kligler iron agar (KIA) (may be replaced by triple sugar iron agar (TSI) but only for enteric pathogens)

1

motility-indole-urease medium (MIU) (may be replaced by motility test medium + peptone urea broth)

1

Kovac’s reagent for indole

1

oxidase reagent

1

Specific identification media and reagents

Biochemical

ONPG test (disc)

2

lysine decarboxylase broth (Mr base)

2

Simmons’ citrate agar

2

Andrade peptone water (or phenol red broth base)

2

acetate agar

3

DNase agar

3

vibriostatic compound 0/129 disc

3

Priority code

Salmonella:

polyvalent 0

1

S. typhi:

anti OD (9, 12), anti H: d, anti Vi

1

S. typhimurium:

anti 0: 4, 5 (B), anti H: i

2

Shigella:

polyvalent flexneri, sonnei, boydii, dysenteriae, specific anti-Shigella dysenteriae 1 (Shiga)

1

Vibrio:

polyvalent 0:1

1

specific anti-ogawa and anti-inaba

3