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close this bookHealth and Environment in Sustainable Development - Five Years after the Earth Summit (WHO, 1991, 128 p.)
close this folderPart II. Essential media and reagents for isolation and identification of clinical pathogens
close this folderPriority grading of pathogens, culture media, and diagnostic reagents
View the document(introduction...)
View the documentBlood culture
View the documentCerebrospinal fluid specimens
View the documentUrine
View the documentStool culture
View the documentLower respiratory tract
View the documentUpper respiratory tract
View the documentUrogenital specimens for agents of sexually transmitted diseases
View the documentPus and exudates
View the documentList of recommended culture media and diagnostic reagents for the intermediate microbiological laboratory

Stool culture

Common pathogens

Pathogens with high priority

Salmonella typhi and S. paratyphi
Shigella
Vibrio cholerae serogroup 01, noncholerae vibrios, Aeromonas and Plesiomonas

Pathogens with intermediate priority

non-typhoid salmonellae and Edwardsiella
Yersinia enterocolitica
Campylobacter jejuni
Clostridium difficile

Pathogens with low priority

Escherichia coli (enteropathogenic, enterotoxigenic, enteroinvasive, and enterohaemorrhagic)

Culture media and diagnostic reagents

Transport media

Priority code

Cary-Blair medium (for all pathogens)

1

buffered glycerol saline (not for Vibrio and Campylobacter)

2

Enrichment media


selenite F broth

2

alkaline peptone water

2

Isolation media


MacConkey agar (with crystal violet)

1

deoxycholate citrate agar (can be replaced by Salmonella-Shigella agar)

1

TCBS agar (can be replaced by Monsur’s TTG agar)

1

Campylobacter medium: Columbia agar base or any blood agar base with lysed blood and antibiotic supplement

2

Clostridium difficile selective medium

3

Preliminary identification media and reagents


Kligler iron agar (KIA) (may be replaced by triple sugar iron agar (TSI) but only for enteric pathogens)

1

motility-indole-urease medium (MIU) (may be replaced by motility test medium + peptone urea broth)

1

Kovac’s reagent for indole

1

oxidase reagent

1

Specific identification media and reagents


Biochemical


ONPG test (disc)

2

lysine decarboxylase broth (Mr base)

2

Simmons’ citrate agar

2

Andrade peptone water (or phenol red broth base)

2

acetate agar

3

DNase agar

3

vibriostatic compound 0/129 disc

3

Serological: agglutinating antisera


Priority code

Salmonella:

polyvalent 0

1

S. typhi:

anti OD (9, 12), anti H: d, anti Vi

1

S. typhimurium:

anti 0: 4, 5 (B), anti H: i

2

Shigella:

polyvalent flexneri, sonnei, boydii, dysenteriae, specific anti-Shigella dysenteriae 1 (Shiga)

1

Vibrio:

polyvalent 0:1

1


specific anti-ogawa and anti-inaba

3