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close this bookHealth and Environment in Sustainable Development - Five Years after the Earth Summit (WHO, 1991, 128 p.)
close this folderPart II. Essential media and reagents for isolation and identification of clinical pathogens
close this folderPriority grading of pathogens, culture media, and diagnostic reagents
View the document(introduction...)
View the documentBlood culture
View the documentCerebrospinal fluid specimens
View the documentUrine
View the documentStool culture
View the documentLower respiratory tract
View the documentUpper respiratory tract
View the documentUrogenital specimens for agents of sexually transmitted diseases
View the documentPus and exudates
View the documentList of recommended culture media and diagnostic reagents for the intermediate microbiological laboratory

Pus and exudates

Common pathogens

Pathogens with high priority

Streptococcus pyogenes
Staphylococcus aureus

Pathogens with intermediate priority

Pseudomonas and other non-fermenters
Clostridium perfringens
Bacteroides and other strict anaerobes
Streptococcus (other species)

Pathogens with low priority

Bacillus anthracis
Mycobacterium tuberculosis, M. ulcerans, etc.
Pasteurella multocida

Culture media and diagnostic reagents

Isolation media

Priority code

blood agar


MacConkey agar


mannitol salt agar


thioglycollate broth (with indicator) (can be replaced by cooked meat medium)


tryptic soy broth (TSB)


Identification media and reagents

coagulase plasma


catalase test (H2O2)


oxidase reagent


hydrogen generator for anaerobic jar