 | Health and Environment in Sustainable Development - Five Years after the Earth Summit (WHO, 1991, 128 p.) |
 |  | Part II. Essential media and reagents for isolation and identification of clinical pathogens |
| | Priority grading of pathogens, culture media, and diagnostic reagents |
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Blood culture
Common pathogens
Pathogens with high priority
Salmonella typhi and non-typhi
other Gram-negative rods
Neisseria meningitidis streptococci (S. pyogenes, S. pneumoniae, viridans streptococci)
Staphylococcus aureus
Pathogens with intermediate priority
Haemophilus influenzae
Bacteroides fragilis
Pseudomonas aeruginosa
Pathogens with low priority
Candida albicans and Cryptococcus neoformans
Brucella
Pseudomonas pseudomallei
non-fermenters other than Pseudomonas aeruginosa
Culture media and diagnostic reagents
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Blood-culture broth |
Priority code | |
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tryptic soy broth (TSB) (can be replaced by any rich broth - for example, brain heart infusion broth) |
1 | |
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optional: addition of sodium polyanethol sulfonate (SPS), 0.25 g per litre “anaerobic” blood-culture broth: |
2 | |
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thioglycollate broth or Schaedler broth or |
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Wilkins-Chalgren anaerobe broth | |
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Isolation media | | |
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subculture on blood agar, chocolate agar, and MacConkey agar |
1 | |
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Identification media and reagents | | |
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oxidase reagent |
1 | |
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optochin disc, bacitracin disc |
1 | |
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b-lactamase test reagent |
1 | |
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V and XV factors |
2 | |
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Neisseria meningitidis agglutinating serum (polyvalent and specific groups A, B, C) |
3 | |
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Haemophilus influenzae type b antiserum |
3 | |
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coagulase plasma |
1 | |
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Salmonella agglutinating antisera |
1 | |
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