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close this bookHealth and Environment in Sustainable Development - Five Years after the Earth Summit (WHO, 1991, 128 p.)
close this folderPart II. Essential media and reagents for isolation and identification of clinical pathogens
close this folderPriority grading of pathogens, culture media, and diagnostic reagents
View the document(introduction...)
View the documentBlood culture
View the documentCerebrospinal fluid specimens
View the documentUrine
View the documentStool culture
View the documentLower respiratory tract
View the documentUpper respiratory tract
View the documentUrogenital specimens for agents of sexually transmitted diseases
View the documentPus and exudates
View the documentList of recommended culture media and diagnostic reagents for the intermediate microbiological laboratory

Upper respiratory tract

Common pathogens

Pathogens with high priority

Streptococcus pyogenes (group A) (throat)
Corynebacterium diphtheriae (throat and nose)
Streptococcus pneumoniae (ear and sinus)
Haemophilus influenzae (ear and sinus)

Pathogens with intermediate priority

Candida albicans (oropharynx)
Staphylococcus aureus (ear and sinus)
Branhamella catarrhalis (ear and sinus)

Pathogens with low priority

Gram-negative rods and Pseudomonas (ear)
Neisseria meningitidis (nasopharyngeal carriers)

Culture media and diagnostic reagents

Isolation media

Priority code

blood agar (prepared from a carbohydrate-free base)


chocolate agar


Ler coagulated serum or Dorset egg medium


tellurite blood agar


modified Thayer-Martin medium (for gonococci and meningococci)


Identification reagents

bacitracin disc, optochin disc


catalase and coagulase reagents


carbohydrate degradation media for Neisseria


V and XV factors (discs or strips)


Rapid diagnostic kits

grouping kit for haemolytic streptococci