|Mushroom Cultivation in Thailand (Peace Corps, 1987, 85 p.)|
A propagation hood is an airtight box in which spore can be moved from one growing medium to another under aseptic conditions (i.e., free of all germs that may attack the pure mushroom culture). The hood should be thoroughly disinfected before each use (use bleach, methyl alcohol or formalin) so that conditions within the hood are truly aseptic. Likewise, all materials to be placed in the box during propagation activities should also be disinfected (i.e., bottles with PDA, alcohol burner, knife, inoculating loop).
Propagation hoods are usually constructed of 1/4-1/2" thick plywood. Dimensions of a hood range from 75-100 cm in length, 50-75 cm in width and 50-75 cm in height.
A large, clear plastic or glass window is placed on the front of the hood at an angle to enable the propagator to view the work in progress.
The hood is equipped with two glove holes in which the hands of the propagator are placed when manipulating propagation equipment to prevent contamination of the boxes contents.
A ventilation door is located on the side or rear of the box. A front door is used to insert and remove propagation equipment.