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close this bookEnergy and Protein requirements, Proceedings of an IDECG workshop, November 1994, London, UK, Supplement of the European Journal of Clinical Nutrition (International Dietary Energy Consultative Group - IDECG, 1994, 198 pages)
close this folderAppendix Criteria for valid nitrogen balance measurement of protein requirements
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View the documentLong-term studies
View the documentAnalysis of multilevel short-term balance studies
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Analysis of multilevel short-term balance studies

Linearity and significance of the nitrogen balance response to increasing levels of nitrogen intake should be verified for each individual's response. For subjects not meeting at least one of these criteria their values should be eliminated from the pooled data and reported separately, with every effort made to repeat the study with such subjects to determine whether the anomalous results were due to experimental error or to some metabolic difference. A regression line, the mean, and 97.5% confidence intervals for the zero-balance intercept should be calculated using the pooled data.