Energy and Protein requirements, Proceedings of an IDECG workshop, November 1994, London, UK, Supplement of the European Journal of Clinical Nutrition (International Dietary Energy Consultative Group - IDECG, 1994, 198 pages): Appendix Criteria for valid nitrogen balance measurement of protein requirements: Long-term studies

Energy and Protein requirements, Proceedings of an IDECG workshop, November 1994, London, UK, Supplement of the European Journal of Clinical Nutrition (International Dietary Energy Consultative Group - IDECG, 1994, 198 pages)

Appendix Criteria for valid nitrogen balance measurement of protein requirements

		(introductory text...)
		Long-term studies
		Analysis of multilevel short-term balance studies
		References

Long-term studies

Conclusions regarding protein needs derived from short-term balance studies should be confirmed by feeding at the proposed safe level for three to six months. It is not practical to conduct the number of long-term studies that would be required to include the many variations in age, sex, physiological state, and genetic and environmental factors. However, several excellent studies established the adequacy of the level of 0.8 g/kg of protein predicted from the mean and confidence limits of the short-term studies (Rand et al, 1984).