Technical notes: Special Considerations for Programming in Unstable Situations (UNICEF, 2000, 490 p.): Chapter 1 - Annex 4: Containing Outbreaks of Cholera, Dysentery and Typhoid: Panels: Panel 4 - Diagnostic Laboratory Supplies for Presumptive Identification of Vibrio Cholerae O1 at a Peripheral Laboratory

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Technical notes: Special Considerations for Programming in Unstable Situations (UNICEF, 2000, 490 p.)

Chapter 1 - Annex 4: Containing Outbreaks of Cholera, Dysentery and Typhoid

Panels

		Panel 1 - Antibiotics Used to Treat Cholera
		Panel 2 - Estimated Minimum Supplies Needed to Treat 100 Patients During a Cholera Outbreak
		Panel 3 - Establishing Emergency Treatment Facilities/Centres
		Panel 4 - Diagnostic Laboratory Supplies for Presumptive Identification of Vibrio Cholerae O1 at a Peripheral Laboratory
		Panel 5 - Some Public Health Supply Requirements

Panel 4 - Diagnostic Laboratory Supplies for Presumptive Identification of Vibrio Cholerae O1 at a Peripheral Laboratory

DIAGNOSTIC LABORATORY SUPPLIES FOR PRESUMPTIVE IDENTIFICATION OF VIBRIO CHOLERAE O1 AT A PERIPHERAL LABORATORY

Specific items for cholera diagnosis

· 100 rectal swabs
· 500 g Cary-Blair medium
· 3 x 300 g TCBS medium or 250 g trypticase, 250 g sodium taurocholate, 2 x 250 g gelatin, 25 g potassium tellurite
· 5 x 2 ml polyvalent O-Group 1 cholera diagnostic antiserum

General laboratory supplies (should already be available in a well-equipped bacterial laboratory)

· 25 g sodium desoxycholate
· 5 g tetramethyl-p-phenylenediamine dihydrochloride
· 250 g Kligler’s iron agar
· 500 g nutrient agar
· 1 kg Bacto-peptone culture medium
· 500 Petri dishes (9 cm)
· 1000 test-tubes (13 x 100 mm)
· 1000 disposable Bijou bottles