Recommended medium

Alternative

Priority code

acetate agar

3

Andrade peptone water

phenol red broth base

2

bile-aesculin agar

1

blood agar base (see tryptic soy agar)

Columbia agar base

1

brolacin agar

purple lactose agar

1

decarboxylase broth base (Mr)

2

DNase agar

3

Kligler iron agar (KIA)

1

Ler coagulated serum

Dorset egg medium

1

Lstein-Jensen medium base

1

MacConkey agar (with crystal violet)

1

mannitol salt agar (MSA)

2

motility-indole-urease medium (MIU)

motility test medium + urea broth + peptone (tryptone) water

1

Mueller-Hinton agar

1

Sabouraud dextrose agar

1

deoxycholate citrate agar

Salmonella-Shigella agar (SS)

1

selenite F broth

2

Simmons’ citrate agar

2

tryptic soy agar (TSA)

1

tryptic soy broth (TSB)

brain heart infusion broth

1

thioglycollate medium (with indicator)

Schaedler broth, Wilkins-Chalgren anaerobe broth, cooked meat medium

2

TCBS

Monsur’s TTG medium

1

transport medium (Amies)

transport medium (Stuart or Cary-Blair)

1

Inhibitors or antibiotics for use in media or as reagents

Priority code

bacitracin (for isolation of Haemophilus spp)

3

Campylobacter antibiotic supplement

2

chloramphenicol (for isolation of fungi)

1

tellurite solution (for isolation of Corynebacterium diphtheriae)

2

vancomycin (for isolation of Haemophilus ducreyi or H. influenzae)

3

gonococcal antibiotic supplement: vancomycin, colistin, nystatin (trimethoprim): VCN (VCNT)

1

Clostridium difficile antibiotic supplement for selective agar

3

Enrichments for culture media

IsoVitaleX (Polyvitex, Vitox, supplement B, supplement VX, supplement CVA)

2

sodium-polyanethol sulfonate (SPS)

3

Diagnostic discs, tablets, or strips

b-glucuronidase (PGUA)

2

bacitracin

1

novobiocin (5 mg)

3

ONPG

1

optochin

1

oxidase

1

V, XV growth factors

2

vibriostatic compound 0/129

3

Nitrocefin disc (Cefinase) or reagent

1

Diagnostic kits

Rapid serodiagnostic kit for identification of meningococci

3

Serological grouping kit for haemolytic streptococci

3

Miscellaneous diagnostic reagents

barium sulfate standard (for Kirby-Bauer method)

1

buffered glycerol-saline (for transportation of stool)

2

Gram stain reagents

1

hydrogen peroxide (for catalase)

1

India ink (for capsule detection)

2

Kovac’s reagent (for indole)

1

oxidase reagent (dimethyl-p-phenylenediamine)

1

plasma (for coagulase test and germ tube test)

1

Ziehl-Neelsen stain

1

carbohydrates: glucose, lactose, maltose, mannitol, sucrose

2

amino acids: lysine (for decarboxylase test)

2

hydrogen generator for anaerobic jar

2

Priority code

Salmonella:

polyvalent o

1

specific O-group: 9, 12 (D) and Vi

1

specific O-group: 4, 5 (B)

2

specific H: d, i

2

Shigella:

polyvalent flexneri, polyvalent boydii, polyvalent

dysenteriae, sonnei

specific: anti-Shigella dysenteriae 1 (Shiga)

1

Vibrio cholerae:

polyvalent 0:1

1

specific: ogawa, inaba

3

Neisseria meningitidis:

polyvalent and group-specific: A, B, C

3

Haemophilus influenzae:

type b

3